209 IL-33 deficiency increases filaggrin protein level with no impact on TEWL increase after tape-stripping

The epidermal barrier function is very important to maintain proper moisture in the human skin and protect inner body homeostasis. stratum corneum (SC) major player function. Interleukin (IL)-33 a tissue-derived cytokine, mainly expressed epithelial endothelial cells, activates Th2 lymphocytes, mast ILC2, eosinophils. We aimed study role of IL-33 steady state. Wild-type (WT), knockout (IL-33KO) mice were tape stripped, trans-epidermal water loss (TEWL) was monitored. Skin samples collected, hematoxylin eosin staining, immunohistochemical (IHC) analyses, western blotting performed. Total RNA extracted from real-time (RT) PCR Tape stripping increased TEWL both IL-33KO WT (p<0.0001) similar levels, there no statistical difference between (n.s.). thickness with an increase granular layer compared mice. observed filaggrin (FLG) staining IHC analyses on days 1, 2, 3 after tape-stripping Tape-stripping induced expression acid ceramidase (ASAH1 ASAH1.2) analysis mice, but not Filaggrin has been implicated function, recently postulated decrease ceramide levels cornified layers atopic dermatitis patients. showed higher protein lower than comparable These results suggest that would be other molecules involved controlling ceramidase.